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5-Bromo-4-chloro-3-indolyl-beta-D-galactoside
Basic Information
- CAS No.: 7240-90-6
- Purity: 99%
Details
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1.What is the 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside ?
White Powder
ChEBI: An indolyl carbohydrate that is the beta-D-galactoside of 3-hydroxy-1H-indole in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for he presence of an enzyme, beta-galactosidase, which cleaved the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole, which immediately dimerises to give an intensely blue product.
5-Bromo-4-chloro-3-indolyl-beta-D-galactoside is a synthetic substrate for β-galactosidase, an enzyme involved in the metabolism of lactose. It is a colorless compound that, upon enzymatic conversion by β-galactosidase, forms an intense indigo-blue chromophore with a maximum absorption at 615 nm. This property makes it a useful tool in various applications, particularly in the field of molecular biology and microbiology.
2.What is the CAS number for 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside ?
The CAS number of 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside is 7240-90-6.
3.What are another words for 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside ?
Synonyms?for?5-Bromo-4-chloro-3-indolyl-beta-D-galactoside 7240-90-6:X-Gal;
4.What is 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside (7240-90-6) used for?
In histochemical staining, in blue-white selection of bacterial colonies with lacZ gene activity during cloning experiments.
InChI:InChI=1/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
Relevant articles related to 5-Bromo-4-chloro-3-indolyl-beta-D-galactoside:
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Synthesis of precipitating chromogenic/fluorogenic β-glucosidase/β-galactosidase substrates by a new method and their application in the visual detection of foodborne pathogenic bacteria |
Wei, Xianhu,Wu, Qingping,Zhang, Jumei,Zhang, Youxiong,Guo, Weipeng,Chen, Moutong,Gu, Qihui,Cai, Zhihe,Lu, Mianfei supporting information, p. 103 - 106 (2016/12/27) |
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Indoxylic acid esters as convenient intermediates towards indoxyl glycosides |
Boettcher, Stephan,Thiem, Joachim , p. 564 - 574 (2014/02/14) |
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Enzymatic synthesis of colorimetric substrates to determine α-2,3- and α-2,6-specific neuraminidase activity |
Reyes Martinez, Juana Elizabeth,Sardzik, Robert,Voglmeir, Josef,Flitsch, Sabine L. , p. 21335 - 21338 (2013/11/06) |
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